Digital PCR for exceptional sensitivity
Digital PCR (dPCR) represents a technological refinement of the conventional qPCR method, providing exceptional sensitivity in detection of low-abundant templates. The crucial step consists in partitioning of the sample to thousands of nanoliter-sized droplets, allowing the PCR reaction to be carried out in each droplet individually. Absolute quantification of target DNA molecules is performed for every single droplet and Poisson statistics is then applied to determine the initial quantity of template in the sample. This enables rare templates to be detected in spite of excessive amounts of other DNA templates.
DigitalTRACE™ - Absolute Quantification of DNA Chimerism
Taking advantage of the dPCR technology, DigitalTRACE™ Indel Assays allow for chimerism monitoring with an unparalleled precision and sensitivity. A range of DigitalTRACE™ HLA Assays is also available for monitoring of the HLA loss events.