QTRACE® Indel Assay

Once recipient specific markers have been found, quantification is performed to monitor engraftment. In the monitoring test, one or more of the informative assays is used to quantify the DNA of interest in an unknown sample relative to a reference sample. Any of the informative assays identified in the genotyping can be used to perform monitoring.

The fraction of DNA positive for the informative marker in the unknown composition is determined relative to the pure reference sample DNA and the result is expressed as a percentage (ratio).




To monitor genomic loss of the mismatched HLA haplotype, a frequent cause of relapse after HSCT, the presence of patient-specific HLA markers is compared to the presence of non HLA markers. HLA loss relapses are characterized by negativity for HLA markers and positivity for non HLA markers.

The HLA TRACE Assays comprise a panel of 19 gene markers covering all major HLA loci (A, B, C, DRB1, DQB1, DPB1). This economic and user-friendly method provides an elegant tool for monitoring of multiple HLA markers in an efficient, comprehensive and affordable manner.