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QTRACE® Indel Assay

Once recipient specific markers have been found, quantification is performed to monitor engraftment. In the monitoring test, one or more of the informative assays is used to quantify the DNA of interest in an unknown sample relative to a reference sample. Any of the informative assays identified in the genotyping can be used to perform monitoring.

The fraction of DNA positive for the informative marker in the unknown composition is determined relative to the pure reference sample DNA and the result is expressed as a percentage (ratio).

 

 
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HLA TRACE Assay

To monitor genomic loss of the mismatched HLA haplotype, a frequent cause of relapse after HSCT, the presence of patient-specific HLA markers is compared to the presence of non HLA markers. HLA loss relapses are characterized by negativity for HLA markers and positivity for non HLA markers.

The HLA TRACE Assays comprise a panel of 19 gene markers covering all major HLA loci (A, B, C, DRB1, DQB1, DPB1). This economic and user-friendly method provides an elegant tool for monitoring of multiple HLA markers in an efficient, comprehensive and affordable manner.

 
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OrganTRACE® Indel Assay

Monitoring of engraftment in solid organ transplant recipients is based on the quantification of donor-derived, cell-free DNA (dd-cfDNA) circulating in the blood. Generally, elevated levels of dd-cfDNA after a certain time period indicate graft failure.

OrganTRACE® Assays are designed for absolute quantification of the level of donor-derived DNA using the highly sensitive digital PCR (dPCR) method that allows for an effective monitoring of engraftment. Longitudinal analysis of the proportion of donor and recipient DNA enables early diagnosis of transplant rejection.

 

 
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DigitalTRACE Indel Assay

The exceptional sensitivity of the digital PCR (dPCR) technology consists in sample partitioning into thousands of nanoliter-sized water-in-oil droplets. Absolute quantification of target DNA molecules is performed for every single droplet within the dPCR tube and this enables rare templates to be detected in presence of excessive amounts of other DNA templates.

Using the dPCR technology, DigitalTRACE Indel Assays allow for chimerism monitoring with an unparalleled precision and sensitivity. A range of DigitalTRACE HLA Assays is also available for monitoring of the HLA loss events.

 
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